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mom kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories mom kit
    Mom Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mom+kit/Mouse+on+Mouse+(M%2EO%2EM%2E+)+Basic+Kit/bio_rxiv__64898__2026__03__03__709336-199-4-6
    Average 96 stars, based on 2773 article reviews
    mom kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Genetics- and age-driven neuroimmune and disc changes underscore herniation susceptibility and pain-associated behaviors in SM/J mice
    Article Snippet: Following antigen retrieval, deparaffinized sections were blocked in 5% normal serum in PBS with Tween 20 and incubated with antibodies against COL2 (1:400, Fitzgerald, 70R-CR008), COMP (1:200, Abcam, ab231977), CS (1:300, Abcam, ab11570), CA3 (1:150, Santa Cruz), IL-6 (1:50, Novus, NB600-1131), and MMP13 (1:150, Abcam, ab39012). .. A MOM kit (Vector Laboratories, BMK-2202) was used for blocking and primary antibody incubation for ARGxx (1:200, Abcam, ab3773) staining. .. Tissue sections were washed and incubated with species-appropriate Alexa Fluor 594–conjugated secondary antibodies (Jackson ImmunoResearch Lab Inc., 1:700).

    Article Title: Sirt6 deficiency promotes senescence and age-associated intervertebral disc degeneration in mice
    Article Snippet: Deparaffinized sections following antigen retrieval or frozen sections were blocked in 5% normal serum in PBS-T, and incubated with antibodies against H3K9Ac (1:50, Sigma, 06-942), collagen I (1:100, Abcam ab34710), collagen X (1:500, Abcam ab58632), chondroitin sulfate (1:300, Abcam ab11570); p21 (1:200, Novus NB100-1941), p19 (1:100, Novus NB200-106), γ-H2AX (1:50, Cell Signaling 9718), IL-6 (1:50, Novus NB600-1131), IL-1β (1:100, Novus NB600-633), MCP1 (1:150, Abcam ab25124), TGF-β (Abcam; ab92486) F-CHP (1:100, 3-Helix). .. For mouse antibodies, a MOM kit (Vector laboratories, BMK-2202) was used for blocking and primary antibody incubation. ..

    Article Title: Sirt6 deficiency promotes senescence and age-associated intervertebral disc degeneration in mice.
    Article Snippet: Immunohistological analyses Deparaffinized sections following antigen retrieval or frozen sections were blocked in 5% normal serum in PBS-T, and incubated with antibodies against H3K9Ac (1:50, Sigma, 06-942), collagen I (1:100, Abcam ab34710), collagen X (1:500, Abcam ab58632), chondroitin sulfate (1:300, Abcam ab11570); p21 (1:200, Novus NB100-1941), p19 (1:100, Novus NB200-106), γ-H2AX (1:50, Cell Signaling 9718), IL-6 (1:50, Novus NB600-1131), IL-1β (1:100, Novus NB600-633), MCP1 (1:150, Abcam ab25124), TGF-β (Abcam; ab92486) F-CHP (1:100, 3- Helix). .. For mouse antibodies, a MOM kit (Vector laboratories, BMK2202) was used for blocking and primary antibody incubation. ..

    Article Title: Targeting the senescence-associated immune checkpoint GD3 ganglioside extends healthspan and blunt age-related diseases with sex-specific benefits
    Article Snippet: For murine tissues, antigen retrieval was performed on 5-μm paraffin sections using a Vector unmasking reagent (Vector Laboratories, ref# H3300). .. Sections were blocked (Avidin/Biotin blocking kit, MOM kit, Vector Laboratories, ref# BMK-2202 and SP-2001) and incubated overnight at 4°C with mouse monoclonal anti-GD3 (Abcam, ref#ab11779, 1:40) or rabbit anti-PCNA (Invitrogen, ref#13-3900, 1:100). .. Detection of primary antibodies involved the use of a biotinylated anti-mouse IgG (MOM kit) or anti-rabbit IgG (Jackson ImmunoResearch, ref# 111-065-144).

    Article Title: Calcitonin receptor downregulation and exercise-conditioned blood enable systemic muscle stem cell proliferation
    Article Snippet: An Alzet mini-osmotic pump (Model 2001), prefilled with 200 μL of -Ex serum with or without IL-6, was implanted under anesthesia. .. For immunohistological analyses, transverse cryosections (6–8 μm) were fixed with 4% paraformaldehyde for 10 min. After blocking with a MOM kit (Vector Laboratories, #BMK-2202), the sections were reacted with anti-Pax7 (clone; Pax7, Developmental Studies Hybridoma Bank, x2), anti-laminin α2 (clone; 4H8-2, Enzo, #ALX-804-190-C100, x200), anti-EHS-laminin (Cosmo Bio, #LB-1013, x1000), anti-CalcR (Bio-Rad, #AHP635, x200), anti-Ki67 (Clone; SolA15, Thermo Fisher Scientific, #14-5698-82, x200), anti-M-cadherin (R&D, #AF4096, x200), or anti-dystrophin (Abcam, #AB15277 x800) antibodies at 4 °C overnight. ..

    Article Title: SIRT6 Activation Improves Intervertebral Disc Health in the Aging Spine
    Article Snippet: Deparaffinized sections following antigen retrieval or frozen sections were blocked in 5% normal serum in PBS-T, and incubated with antibodies against H3K9Ac (1:50, Sigma, 06-942), Collagen I (1:100, Abcam ab34710), Collagen X (1:500, Abcam ab58632), Aggrecan (1:300, Abcam ab11570); GLUT1 (1:200, Abcam, ab40084), F-CHP (1:100, 3-Helix), p21 (1:200, Novus NB100-1941), IL-6 (1:50, Novus NB600-1131), TGF-ý (1:200, Abcam; ab92486) (n= 4-6 mice/group, 2-3 discs/mouse were analyzed). .. For mouse antibodies, a MOM kit (Vector Laboratories, BMK-2202) was used for blocking and primary antibody incubation. ..

    Incubation:

    Article Title: Genetics- and age-driven neuroimmune and disc changes underscore herniation susceptibility and pain-associated behaviors in SM/J mice
    Article Snippet: Following antigen retrieval, deparaffinized sections were blocked in 5% normal serum in PBS with Tween 20 and incubated with antibodies against COL2 (1:400, Fitzgerald, 70R-CR008), COMP (1:200, Abcam, ab231977), CS (1:300, Abcam, ab11570), CA3 (1:150, Santa Cruz), IL-6 (1:50, Novus, NB600-1131), and MMP13 (1:150, Abcam, ab39012). .. A MOM kit (Vector Laboratories, BMK-2202) was used for blocking and primary antibody incubation for ARGxx (1:200, Abcam, ab3773) staining. .. Tissue sections were washed and incubated with species-appropriate Alexa Fluor 594–conjugated secondary antibodies (Jackson ImmunoResearch Lab Inc., 1:700).

    Article Title: Sirt6 deficiency promotes senescence and age-associated intervertebral disc degeneration in mice
    Article Snippet: Deparaffinized sections following antigen retrieval or frozen sections were blocked in 5% normal serum in PBS-T, and incubated with antibodies against H3K9Ac (1:50, Sigma, 06-942), collagen I (1:100, Abcam ab34710), collagen X (1:500, Abcam ab58632), chondroitin sulfate (1:300, Abcam ab11570); p21 (1:200, Novus NB100-1941), p19 (1:100, Novus NB200-106), γ-H2AX (1:50, Cell Signaling 9718), IL-6 (1:50, Novus NB600-1131), IL-1β (1:100, Novus NB600-633), MCP1 (1:150, Abcam ab25124), TGF-β (Abcam; ab92486) F-CHP (1:100, 3-Helix). .. For mouse antibodies, a MOM kit (Vector laboratories, BMK-2202) was used for blocking and primary antibody incubation. ..

    Article Title: Sirt6 deficiency promotes senescence and age-associated intervertebral disc degeneration in mice.
    Article Snippet: Immunohistological analyses Deparaffinized sections following antigen retrieval or frozen sections were blocked in 5% normal serum in PBS-T, and incubated with antibodies against H3K9Ac (1:50, Sigma, 06-942), collagen I (1:100, Abcam ab34710), collagen X (1:500, Abcam ab58632), chondroitin sulfate (1:300, Abcam ab11570); p21 (1:200, Novus NB100-1941), p19 (1:100, Novus NB200-106), γ-H2AX (1:50, Cell Signaling 9718), IL-6 (1:50, Novus NB600-1131), IL-1β (1:100, Novus NB600-633), MCP1 (1:150, Abcam ab25124), TGF-β (Abcam; ab92486) F-CHP (1:100, 3- Helix). .. For mouse antibodies, a MOM kit (Vector laboratories, BMK2202) was used for blocking and primary antibody incubation. ..

    Article Title: Targeting the senescence-associated immune checkpoint GD3 ganglioside extends healthspan and blunt age-related diseases with sex-specific benefits
    Article Snippet: For murine tissues, antigen retrieval was performed on 5-μm paraffin sections using a Vector unmasking reagent (Vector Laboratories, ref# H3300). .. Sections were blocked (Avidin/Biotin blocking kit, MOM kit, Vector Laboratories, ref# BMK-2202 and SP-2001) and incubated overnight at 4°C with mouse monoclonal anti-GD3 (Abcam, ref#ab11779, 1:40) or rabbit anti-PCNA (Invitrogen, ref#13-3900, 1:100). .. Detection of primary antibodies involved the use of a biotinylated anti-mouse IgG (MOM kit) or anti-rabbit IgG (Jackson ImmunoResearch, ref# 111-065-144).

    Article Title: SIRT6 Activation Improves Intervertebral Disc Health in the Aging Spine
    Article Snippet: Deparaffinized sections following antigen retrieval or frozen sections were blocked in 5% normal serum in PBS-T, and incubated with antibodies against H3K9Ac (1:50, Sigma, 06-942), Collagen I (1:100, Abcam ab34710), Collagen X (1:500, Abcam ab58632), Aggrecan (1:300, Abcam ab11570); GLUT1 (1:200, Abcam, ab40084), F-CHP (1:100, 3-Helix), p21 (1:200, Novus NB100-1941), IL-6 (1:50, Novus NB600-1131), TGF-ý (1:200, Abcam; ab92486) (n= 4-6 mice/group, 2-3 discs/mouse were analyzed). .. For mouse antibodies, a MOM kit (Vector Laboratories, BMK-2202) was used for blocking and primary antibody incubation. ..

    Staining:

    Article Title: Genetics- and age-driven neuroimmune and disc changes underscore herniation susceptibility and pain-associated behaviors in SM/J mice
    Article Snippet: Following antigen retrieval, deparaffinized sections were blocked in 5% normal serum in PBS with Tween 20 and incubated with antibodies against COL2 (1:400, Fitzgerald, 70R-CR008), COMP (1:200, Abcam, ab231977), CS (1:300, Abcam, ab11570), CA3 (1:150, Santa Cruz), IL-6 (1:50, Novus, NB600-1131), and MMP13 (1:150, Abcam, ab39012). .. A MOM kit (Vector Laboratories, BMK-2202) was used for blocking and primary antibody incubation for ARGxx (1:200, Abcam, ab3773) staining. .. Tissue sections were washed and incubated with species-appropriate Alexa Fluor 594–conjugated secondary antibodies (Jackson ImmunoResearch Lab Inc., 1:700).

    Avidin-Biotin Assay:

    Article Title: Targeting the senescence-associated immune checkpoint GD3 ganglioside extends healthspan and blunt age-related diseases with sex-specific benefits
    Article Snippet: For murine tissues, antigen retrieval was performed on 5-μm paraffin sections using a Vector unmasking reagent (Vector Laboratories, ref# H3300). .. Sections were blocked (Avidin/Biotin blocking kit, MOM kit, Vector Laboratories, ref# BMK-2202 and SP-2001) and incubated overnight at 4°C with mouse monoclonal anti-GD3 (Abcam, ref#ab11779, 1:40) or rabbit anti-PCNA (Invitrogen, ref#13-3900, 1:100). .. Detection of primary antibodies involved the use of a biotinylated anti-mouse IgG (MOM kit) or anti-rabbit IgG (Jackson ImmunoResearch, ref# 111-065-144).



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